Exploitation of Laminar Flow for Cell-Based Assays

M. F. Santillo1, and A. G. Ewing1,2
1Department of Chemistry, Pennsylvania State University, University Park, PA, USA
2Department of Chemistry, Göteborg University, Göteborg, Sweden

A microfluidic device for performing multiplexed cell bioassays has been successfully developed. The device takes advantage of laminar flow and hydrodynamic focusing in order to selectively load cells into various culture chambers and address cells with pharmacological agents.

Both computational fluid dynamics (CFD) simulations and fluorescence microscopy illustrate how fluid flow can be manipulated, which is central to the function of this device.