Modeling Two-Phase Electrophoresis

W. Clark[1], and M. Lindblad[1]
[1]Chemical Engineering Department, Worcester Polytechnic Institute, Worcester, Massachusetts, USA

Two-phase electrophoresis is a separation method that combines aqueous two-phase partitioning with electrophoresis and has promise for large scale recovery of biological products. Aqueous two-phase systems formed by adding two polymers, like dextran and polyethylene glycol, to water provide some separation of dissolved species due to differences in solubility of solutes between the phases. COMSOL Multiphysics is well suited to handle this complex problem. We have demonstrated two-phase electrophoresis separations of proteins in both batch and flow devices.