Local Electroporation of Single Adherent Cells by Micro-Structured Needle ElectrodesK. K. Sriperumbudur, P. J. Koester, M. Stubbe, C. Tautorat, J. Held, W. Baumann, and J. Gimsa
 University of Rostock, Chair of Biophysics, Gertrudenstr. 11a, 18057 Rostock, Germany
 Microsystem Material Laboratory, Department of Microsystems Engineering (IMTEK), University of Freiburg, Germany
In spite of its low throughput, Patch-Clamp is the established method for intracellular measurements of the transmembrane potential. To address this problem, we have developed new biosensor-chips with micro-structured needle electrodes (MNEs). MNE-penetration of single cells growing on the MNE-tips leads to a situation comparable to the whole-cell mode in classical Patch Clamp. MNE-penetration was accomplished by local micro-invasive needle electroporation (LOMINE; Koester et al., 2008; Baumann et al., 1998a and b). In this paper, we simulate the field and potential distributions around the MNE before LOMINE assuming reasonable cell and medium parameters for a cell being in contact with the needle via focal adhesion points.