Chemical Reactions in a Microfluidic T-Sensor: Numerical Comparison of 2D and 3D Models

R. Winz[1][2], N. Schröder[1], W. Wiechert[1], and E. von Lieres[1]
[1]Institute of Biotechnology 2, Research Centre Jülich, Jülich, Germany
[2]Research Center for Micro and Nanochemistry, University of Siegen, Siegen, Germany

In recent years lab-on-microchip technology has become a powerful tool for micro-scale analysis of biochemical processes. In the studied system the overall process consists of transport, convection, diffusion, reaction and adsorption processes. Two compounds A and B, contained in a carrier fluid (buffer), are introduced into a reaction channel via a Y-shaped double-inlet. As the streams flow laminary side by side, inter-diffusion allows for mixing of the two compounds. While mixing, these two compounds react to form a third complex C. It is only the latter compound that is then adsorbed at a functionalized surface, coated with receptors to form a complex bond (donor-acceptor-bond). The amount of adsorbed molecules C’ can be quantified via an increase in fluorescence.

Download