Microfluidic Systems for Cell Growth and Cell Migration Studies

M.Dimaki[1], P. Shah[1], D. Kwasny[1], J. Lange Moresco[2], and W. E. Svendsen[1]
[1]Department of Micro- and Nanotechnology, Technical University of Denmark, Lyngby, Denmark
[2]Sunstone Capital, Copenhagen, Denmark

Lab-on-a-chip devices for cell culturing are becoming imperative in order to satisfy the needs for point-of-care or decentralized laboratory testing. Towards that goal we have developed miniaturized membrane based cell culture chambers for use within the field of chromosome analysis. The same principle was applied for the development of a miniaturized cell migration device.

Flow and concentration gradient simulations have been conducted in order to evaluate the performance of the devices before their fabrication but also in order to confirm and explain the experimental results. This paper will present the idea behind membrane based culturing and the resulting designs, as well as the theoretical and experimental results.