Chemical Reactions in a Microfluidic T-Sensor: Numerical Comparison of 2D and 3D ModelsR. Winz, N. Schröder, W. Wiechert, and E. von Lieres
Institute of Biotechnology 2, Research Centre Jülich, Jülich, Germany
Research Center for Micro and Nanochemistry, University of Siegen, Siegen, Germany
In recent years lab-on-microchip technology has become a powerful tool for micro-scale analysis of biochemical processes. In the studied system the overall process consists of transport, convection, diffusion, reaction and adsorption processes. Two compounds A and B, contained in a carrier fluid (buffer), are introduced into a reaction channel via a Y-shaped double-inlet. As the streams flow laminary side by side, inter-diffusion allows for mixing of the two compounds. While mixing, these two compounds react to form a third complex C. It is only the latter compound that is then adsorbed at a functionalized surface, coated with receptors to form a complex bond (donor-acceptor-bond). The amount of adsorbed molecules C’ can be quantified via an increase in fluorescence.