Zone Electrophoresis

Application ID: 47641

Zone electrophoresis (ZE) is an electrophoretic separation technique typically used for analyzing proteins, nucleic acids, and biopolymers. During the process, different species in a sample are transported in a continuous electrolyte buffer system, subject to a potential gradient. Due to differences in the mobilities, the species in the samples will eventually separate into different, well-resolved peaks.

This tutorial introduces the Electrophoretic Transport interface. A buffer system consisting of an acetic acid and tris(tris(hydroxymethyl)aminomethane) is used to separate a sample consisting of pyridine and aniline into two well-resolved peaks.

This model example illustrates applications of this type that would nominally be built using the following products: